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KMID : 0624620080410040305
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BMB Reports
2008 Volume.41 No. 4 p.305 ~ p.309
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Effect of metal ions on the secondary structure and activity of calf intestine phosphatase
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Chen Fengjuan
Liu Guoqi
Xu Zhihong
Zeng Zhengzhi
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Abstract
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Cobalt is an essential microelements in many biological processes involving enzymatic activity. We found that Zn2+ and Mg2+, which are in the active site of native calf intestine alkaline phosphatase (CIP), can be replaced by Co2+ directly in solution. The effect of Co2+ concentration on the substitution reaction was examined at ratios of [Co2+]/[CIP] from 0:1 to 8:1. The quantity of Zn2+ in CIP decreased progressively as the ratio was increased, but the amount of Mg2+ changed in irrregular fashion. A series of active site models of the reaction mechanism of CIP are proposed. Low pH was found to promote the replacement of Mg2+ by Co2+. To understand how the substitution affects the enzyme, we also solved the secondary structure of CIP after reaction with Co2+ in different conditions. [BMB reports 2008; 41(4): 305-309]
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KEYWORD
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Activity, Calf intestine alkaline phosphatase, Cobalt ions, Secondary structure
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